A metal-binding member of the late embryogenesis abundant protein family transports iron in the phloem of Ricinus communis L.
Identifieur interne : 000407 ( Main/Exploration ); précédent : 000406; suivant : 000408A metal-binding member of the late embryogenesis abundant protein family transports iron in the phloem of Ricinus communis L.
Auteurs : Claudia Kruger [Allemagne] ; Oliver Berkowitz ; Udo W. Stephan ; Rudiger HellSource :
- The Journal of biological chemistry [ 0021-9258 ] ; 2002.
Descripteurs français
- KwdFr :
- Amorces ADN (MeSH), Données de séquences moléculaires (MeSH), Fer (métabolisme), Graines (MeSH), Protéines de transport (composition chimique), Protéines de transport (métabolisme), Protéines végétales (composition chimique), Protéines végétales (métabolisme), Ricinus (embryologie), Ricinus (métabolisme), Similitude de séquences d'acides aminés (MeSH), Séquence d'acides aminés (MeSH), Séquence nucléotidique (MeSH), Transport des ions (MeSH), Zinc (métabolisme).
- MESH :
- composition chimique : Protéines de transport, Protéines végétales.
- embryologie : Ricinus.
- métabolisme : Fer, Protéines de transport, Protéines végétales, Ricinus, Zinc.
- Amorces ADN, Données de séquences moléculaires, Graines, Similitude de séquences d'acides aminés, Séquence d'acides aminés, Séquence nucléotidique, Transport des ions.
English descriptors
- KwdEn :
- Amino Acid Sequence (MeSH), Base Sequence (MeSH), Carrier Proteins (chemistry), Carrier Proteins (metabolism), DNA Primers (MeSH), Ion Transport (MeSH), Iron (metabolism), Molecular Sequence Data (MeSH), Plant Proteins (chemistry), Plant Proteins (metabolism), Ricinus (embryology), Ricinus (metabolism), Seeds (MeSH), Sequence Homology, Amino Acid (MeSH), Zinc (metabolism).
- MESH :
- chemical , chemistry : Carrier Proteins, Plant Proteins.
- chemical , metabolism : Carrier Proteins, Iron, Plant Proteins, Zinc.
- embryology : Ricinus.
- metabolism : Ricinus.
- Amino Acid Sequence, Base Sequence, DNA Primers, Ion Transport, Molecular Sequence Data, Seeds, Sequence Homology, Amino Acid.
Abstract
The transport of metal micronutrients to developing organs in a plant is mediated primarily by the sieve elements. Ligands are thought to form complexes with the free ions in order to prevent cellular damage, but no binding partners have been unequivocally identified from plants so far. This study has used the phloem-mediated transport of micronutrients during the germination of the castor bean seedling to identify an iron transport protein (ITP). It is demonstrated that essentially all (55)Fe fed to seedlings is associated with the protein fraction of phloem exudate. It is shown that ITP carries iron in vivo and binds additional iron in vitro. ITP was purified to homogeneity from minute amounts of phloem exudate using immobilized metal ion affinity chromatography. It preferentially binds to Fe(3+) but not to Fe(2+) and also complexes Cu(2+), Zn(2+), and Mn(2+) in vitro. The corresponding cDNA of ITP was cloned using internal peptide fragments. The deduced protein of 96 amino acids shows high similarity to the stress-related family of late embryogenesis abundant proteins. Its predicted characteristics and its RNA expression pattern are consistent with a function in metal ion binding. The ITP from Ricinus provides the first identified micronutrient binding partner for phloem-mediated long distance transport in plants and is the first member of the late embryogenesis abundant protein family shown to have such a function.
DOI: 10.1074/jbc.M201896200
PubMed: 11983700
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Stephan</name></author><author><name sortKey="Hell, Rudiger" sort="Hell, Rudiger" uniqKey="Hell R" first="Rudiger" last="Hell">Rudiger Hell</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2002">2002</date><idno type="RBID">pubmed:11983700</idno><idno type="pmid">11983700</idno><idno type="doi">10.1074/jbc.M201896200</idno><idno type="wicri:Area/Main/Corpus">000402</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000402</idno><idno type="wicri:Area/Main/Curation">000402</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000402</idno><idno type="wicri:Area/Main/Exploration">000402</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">A metal-binding member of the late embryogenesis abundant protein family transports iron in the phloem of Ricinus communis L.</title><author><name sortKey="Kruger, Claudia" sort="Kruger, Claudia" uniqKey="Kruger C" first="Claudia" last="Kruger">Claudia Kruger</name><affiliation wicri:level="1"><nlm:affiliation>Institut für Pflanzengenetik und Kulturpflanzenforschung Gatersleben (Leibniz-Institut), Corrensstrasse 3, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institut für Pflanzengenetik und Kulturpflanzenforschung Gatersleben (Leibniz-Institut), Corrensstrasse 3</wicri:regionArea><wicri:noRegion>Corrensstrasse 3</wicri:noRegion><wicri:noRegion>Corrensstrasse 3</wicri:noRegion><wicri:noRegion>Corrensstrasse 3</wicri:noRegion></affiliation></author><author><name sortKey="Berkowitz, Oliver" sort="Berkowitz, Oliver" uniqKey="Berkowitz O" first="Oliver" last="Berkowitz">Oliver Berkowitz</name></author><author><name sortKey="Stephan, Udo W" sort="Stephan, Udo W" uniqKey="Stephan U" first="Udo W" last="Stephan">Udo W. Stephan</name></author><author><name sortKey="Hell, Rudiger" sort="Hell, Rudiger" uniqKey="Hell R" first="Rudiger" last="Hell">Rudiger Hell</name></author></analytic><series><title level="j">The Journal of biological chemistry</title><idno type="ISSN">0021-9258</idno><imprint><date when="2002" type="published">2002</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence (MeSH)</term><term>Base Sequence (MeSH)</term><term>Carrier Proteins (chemistry)</term><term>Carrier Proteins (metabolism)</term><term>DNA Primers (MeSH)</term><term>Ion Transport (MeSH)</term><term>Iron (metabolism)</term><term>Molecular Sequence Data (MeSH)</term><term>Plant Proteins (chemistry)</term><term>Plant Proteins (metabolism)</term><term>Ricinus (embryology)</term><term>Ricinus (metabolism)</term><term>Seeds (MeSH)</term><term>Sequence Homology, Amino Acid (MeSH)</term><term>Zinc (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Amorces ADN (MeSH)</term><term>Données de séquences moléculaires (MeSH)</term><term>Fer (métabolisme)</term><term>Graines (MeSH)</term><term>Protéines de transport (composition chimique)</term><term>Protéines de transport (métabolisme)</term><term>Protéines végétales (composition chimique)</term><term>Protéines végétales (métabolisme)</term><term>Ricinus (embryologie)</term><term>Ricinus (métabolisme)</term><term>Similitude de séquences d'acides aminés (MeSH)</term><term>Séquence d'acides aminés (MeSH)</term><term>Séquence nucléotidique (MeSH)</term><term>Transport des ions (MeSH)</term><term>Zinc (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Carrier Proteins</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Carrier Proteins</term><term>Iron</term><term>Plant Proteins</term><term>Zinc</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Protéines de transport</term><term>Protéines végétales</term></keywords><keywords scheme="MESH" qualifier="embryologie" xml:lang="fr"><term>Ricinus</term></keywords><keywords scheme="MESH" qualifier="embryology" xml:lang="en"><term>Ricinus</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Ricinus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Fer</term><term>Protéines de transport</term><term>Protéines végétales</term><term>Ricinus</term><term>Zinc</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Base Sequence</term><term>DNA Primers</term><term>Ion Transport</term><term>Molecular Sequence Data</term><term>Seeds</term><term>Sequence Homology, Amino Acid</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Amorces ADN</term><term>Données de séquences moléculaires</term><term>Graines</term><term>Similitude de séquences d'acides aminés</term><term>Séquence d'acides aminés</term><term>Séquence nucléotidique</term><term>Transport des ions</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The transport of metal micronutrients to developing organs in a plant is mediated primarily by the sieve elements. Ligands are thought to form complexes with the free ions in order to prevent cellular damage, but no binding partners have been unequivocally identified from plants so far. This study has used the phloem-mediated transport of micronutrients during the germination of the castor bean seedling to identify an iron transport protein (ITP). It is demonstrated that essentially all (55)Fe fed to seedlings is associated with the protein fraction of phloem exudate. It is shown that ITP carries iron in vivo and binds additional iron in vitro. ITP was purified to homogeneity from minute amounts of phloem exudate using immobilized metal ion affinity chromatography. It preferentially binds to Fe(3+) but not to Fe(2+) and also complexes Cu(2+), Zn(2+), and Mn(2+) in vitro. The corresponding cDNA of ITP was cloned using internal peptide fragments. The deduced protein of 96 amino acids shows high similarity to the stress-related family of late embryogenesis abundant proteins. Its predicted characteristics and its RNA expression pattern are consistent with a function in metal ion binding. The ITP from Ricinus provides the first identified micronutrient binding partner for phloem-mediated long distance transport in plants and is the first member of the late embryogenesis abundant protein family shown to have such a function.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">11983700</PMID><DateCompleted><Year>2002</Year><Month>08</Month><Day>12</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0021-9258</ISSN><JournalIssue CitedMedium="Print"><Volume>277</Volume><Issue>28</Issue><PubDate><Year>2002</Year><Month>Jul</Month><Day>12</Day></PubDate></JournalIssue><Title>The Journal of biological chemistry</Title><ISOAbbreviation>J Biol Chem</ISOAbbreviation></Journal><ArticleTitle>A metal-binding member of the late embryogenesis abundant protein family transports iron in the phloem of Ricinus communis L.</ArticleTitle><Pagination><MedlinePgn>25062-9</MedlinePgn></Pagination><Abstract><AbstractText>The transport of metal micronutrients to developing organs in a plant is mediated primarily by the sieve elements. Ligands are thought to form complexes with the free ions in order to prevent cellular damage, but no binding partners have been unequivocally identified from plants so far. This study has used the phloem-mediated transport of micronutrients during the germination of the castor bean seedling to identify an iron transport protein (ITP). It is demonstrated that essentially all (55)Fe fed to seedlings is associated with the protein fraction of phloem exudate. It is shown that ITP carries iron in vivo and binds additional iron in vitro. ITP was purified to homogeneity from minute amounts of phloem exudate using immobilized metal ion affinity chromatography. It preferentially binds to Fe(3+) but not to Fe(2+) and also complexes Cu(2+), Zn(2+), and Mn(2+) in vitro. The corresponding cDNA of ITP was cloned using internal peptide fragments. The deduced protein of 96 amino acids shows high similarity to the stress-related family of late embryogenesis abundant proteins. Its predicted characteristics and its RNA expression pattern are consistent with a function in metal ion binding. The ITP from Ricinus provides the first identified micronutrient binding partner for phloem-mediated long distance transport in plants and is the first member of the late embryogenesis abundant protein family shown to have such a function.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Kruger</LastName><ForeName>Claudia</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Institut für Pflanzengenetik und Kulturpflanzenforschung Gatersleben (Leibniz-Institut), Corrensstrasse 3, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Berkowitz</LastName><ForeName>Oliver</ForeName><Initials>O</Initials></Author><Author ValidYN="Y"><LastName>Stephan</LastName><ForeName>Udo W</ForeName><Initials>UW</Initials></Author><Author ValidYN="Y"><LastName>Hell</LastName><ForeName>Rudiger</ForeName><Initials>R</Initials></Author></AuthorList><Language>eng</Language><DataBankList 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UI="D007501">Iron</NameOfSubstance></Chemical><Chemical><RegistryNumber>J41CSQ7QDS</RegistryNumber><NameOfSubstance UI="D015032">Zinc</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002352" MajorTopicYN="N">Carrier Proteins</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017931" MajorTopicYN="N">DNA Primers</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017136" MajorTopicYN="N">Ion Transport</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007501" MajorTopicYN="N">Iron</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012278" MajorTopicYN="N">Ricinus</DescriptorName><QualifierName UI="Q000196" MajorTopicYN="N">embryology</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012639" MajorTopicYN="N">Seeds</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017386" MajorTopicYN="N">Sequence Homology, Amino Acid</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015032" MajorTopicYN="N">Zinc</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2002</Year><Month>5</Month><Day>2</Day><Hour>10</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2002</Year><Month>8</Month><Day>13</Day><Hour>10</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2002</Year><Month>5</Month><Day>2</Day><Hour>10</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">11983700</ArticleId><ArticleId IdType="doi">10.1074/jbc.M201896200</ArticleId><ArticleId IdType="pii">M201896200</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Allemagne</li></country></list><tree><noCountry><name sortKey="Berkowitz, Oliver" sort="Berkowitz, Oliver" uniqKey="Berkowitz O" first="Oliver" last="Berkowitz">Oliver Berkowitz</name><name sortKey="Hell, Rudiger" sort="Hell, Rudiger" uniqKey="Hell R" first="Rudiger" last="Hell">Rudiger Hell</name><name sortKey="Stephan, Udo W" sort="Stephan, Udo W" uniqKey="Stephan U" first="Udo W" last="Stephan">Udo W. Stephan</name></noCountry><country name="Allemagne"><noRegion><name sortKey="Kruger, Claudia" sort="Kruger, Claudia" uniqKey="Kruger C" first="Claudia" last="Kruger">Claudia Kruger</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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